Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Genome studied.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques:

Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Phylogenomic analysis among Fonsecaea sibling species. (A) Phylogenetic tree based on the orthologous gene clusters. The bantiana-clade is indicated by a red line and the carrionii-clade by a blue line. Species names in green boxes are environmental strains, in red boxes agents of chromoblastomycosis and cerebral infection. (B) Gene cluster distribution in Fonsecaea siblings. Clusters of orthologous genes are showed in colors: dark gray core genes; light gray accessory genes, black specific genes. 1. F. monophora CBS 269.37, 2. F. multimorphosa CBS 980.96. 3. F. multimorphosa CBS 102226, 4. F. pedrosoi CBS 271.37, 5. F. nubica CBS 269.64, 6. F. erecta CBS 125763.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques: Infection

Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Fonsecaea and Cladophialophora specific genes annotated in the PHI base.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques: Infection, Activity Assay

Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Virulence of Fonsecaea siblings using Galleria mellonella larvae. (A) Survival of G. mellonella larvae infected with Fonsecaea species. (B) Melanization of G. mellonella larvae infected with Fonsecaea species: (1, 2). Controls: SHAM and PBS; (3). Fonsecaea erecta CBS 125763; (4). Fonsecaea monophora ; (5). Fonsecaea pedrosoi CBS 271.37; (6). Fonsecaea pedrosoi ATCC 46428. (C) Histology of infected tissue of G. mellonella with Fonsecaea species. The internal structures were fixed, embedded in paraffin and stained with PAS. Black arrows show hyphae spreading through the larva tissue. (1, 2). Fonsecaea erecta CBS 125763; (3, 4). Fonsecaea pedrosoi CBS 271.37 ; (5, 6). Fonsecaea monophora CBS 102248.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques: Infection, Staining

Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Fungal burden and production of pro-inflammatory cytokines of F. pedrosoi and F. erecta . (A) J774 murine macrophages (J774) were co-cultured with conidia or hyphal fragments, in the MOI 1 during 24 h. CFU data showed faster clearance of inoculated conidia from F. pedrosoi than F. erecta . (B) High levels of TNF-α were observed in macrophage co-culture with hypha, but not with conidia. Data were analyzed by one-way ANOVA with Tukey's post-hoc test. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with F. pedrosoi ; or compared with non-infected macrophages.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques: Cell Culture, Co-Culture Assay, Infection

Journal: Frontiers in Microbiology

Article Title: Comparative Genomics of Sibling Species of Fonsecaea Associated with Human Chromoblastomycosis

doi: 10.3389/fmicb.2017.01924

Figure Lengend Snippet: Virulence and immunostimulatory potential test of Fonsecaea sibling species using BALB/c mice as a model. (A) Macroscopic aspect of the disease. (B) CFU data showed a high tissue fungal burden of F. erecta which decline over the course of the infection. (C–F) At 7 and 14 days post-infection, high levels of TNF-α, IL-1β, IL-6 and MCP-1 were observed similarly in the footpad of mice infected with F. pedrosoi or F. erecta . Data were analyzed by two-way ANOVA with Tukey's post-hoc test. * p < 0.05 and ** p < 0.01 between groups indicated by brackets; n.s.: not significantly.

Article Snippet: The above hypothesis is partially supported by results of virulence testing using G. mellonella larvae as a model. Larvae infected by environmental F. erecta had a lower survival than those infected by clinical strains of F. pedrosoi (ATCC 46428 and CBS 271.37 T ) and F. monophora (CBS 102248).

Techniques: Infection